We are interested in studying gene functions using Caenorhabditis
elegans as an animal model system. C. elegans is an excellent model
organism in laboratory to study gene functions since it has a short
life cycle as 3 days from an egg to an adult and its genome has
been completely sequenced. Developmental study on C. elegans is
powerful because its cell lineage has been well confirmed. A genetic
approach using C. elegans is also a powerful tool to study because
it can be either hermaphrodite or male, and massive mutant library
is well established. Most importantly, 74% of known human genes
are also found in C. elegans and 40% of them have high similarities.
Therefore, C. elegans is a good model system to study human gene
functions by using its simplicity and other powerful tools for investigating
gene functions. In our laboratory, we are currently focusing to
three different projects:
1. Functional genomics of genes for germ-line proliferation in
2. Functional genomics of cholesterol-related genes in C. elegans
3. Functional genomics of caffeine-induced genes in C. elegans
Lee YU, Kim J, Son MS, Shim YH, Kawasaki I.
CDC-25.2, a C. elegans ortholog of cdc25, is essential for the progression of intestinal divisions.
Cell Cycle (2016, in press)
Min H, Shim YH, Kawasaki I. PGL-1 and PGL-3, a family of constitutive P-granule components, prevent excessive levels of germline apoptosis in Caenorhabditis elegans.
J Cell Sci 129:341-353 (2016)
Mohammad Al-Amin, Gong JM, Kawasaki I, Shim YH. Caffeine induces the stress response and up-regulates heat shock proteins in Caenorhabditis elegans.
Mol Cells ( 2016, in press)
Min H, Kawasaki I, Gong JM, Shim YH. Caffeine induces high expression of cyp-35A family genes and inhibits the early larval development in Caenorhabditis elegans.
Mol Cells 38(3): 236-242 (2015)
Ko S, Kawasaki I, Shim YH. PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1.
PLOS ONE 8:e84798 (2013)
Yoon SH, Kawasaki I, Shim YH. CDC-25.1 controls the rate of germline mitotic cell cycle by counteracting WEE-1.3 and by positively regulating CDK-1 in Caenorhabditis elegans.
Cell Cycle 11:1354-1363 (2012)
Kim J, Kawasaki I, Shim YH. A cdc25 ortholog, cdc-25.2 promotes oocyte maturation in C. elegans.
J of Cell Sci 123: 1-10 (2010).
Shim YH, Paik YK. Caenorhabditis elegans proteomics comes of age. Proteomics 10(4):846-857 (2010
Do-Hwan Ahn, Gunasekaran Singaravelu,
Sooung Lee, Joohong Ahnn, and Yhong-Hee Shim.
Functional and phenotypic relevance of differentially expressed proteins
in calcineurin mutants of Caenorhabditis elegans. Proteomics 6:1340-1350 (2006).