AG Lab

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Caffeine inhibits the early larval development
 심용희  | 2015·11·19 16:48 | HIT : 877 | VOTE : 38 |
Intake of caffeine during pregnancy can cause fetal developmental retardation. Although significant influence of caffeine on animal development is widely recognized, its mode of action remains little known because of its pleiotropic effects on living organisms. In this study, by using Caenorhabditis elegans as a model organism, we investigated the effects of caffeine during the development at both embryonic and post-embryonic stages. We examined brood size, embryonic lethality, and percent larval development, and found that caffeine inhibits C. elegans development at most of the stages in dosage-dependent fashion. Upon 30 mM caffeine treatment, majority (86.1 ± 3.4%) of the newly hatched L1 larvae were irreversibly arrested without further development. In contrast, many of the late-stage larvae survived and grew to adults on the same 30 mM caffeine-containing plates. These results suggest that early-stage larvae are more susceptible to caffeine than later-stage larvae. To understand metabolic responses to caffeine treatment, we examined expression levels of cytochrome p450 (cyp) genes with or without caffeine treatment by using comparative microarray and found that expression of 24 cyp genes was more than 2-fold (p < 0.05) increased by caffeine treatment. Among them, induction of cyp-35A gene family was most prominent. Interestingly, depletion of cyp-35A family genes one-by-one or in combination by RNA interference resulted in partial rescue from early larval developmental arrest caused by caffeine treatment, suggesting that overexpression of cyp-35A family genes can be fatal to development of early-stage larvae.
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